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Current issue

ELEKTRO 5/2019 was released on May 15th 2019. Its digital version will be available imediately.

Topic: Lightning and overvoltage protection; Fire and safety technologies

Main Article
Verification of material coefficient defined in the standard STN EN 62305-3
Smart Cities (final part 10)

SVĚTLO (Light) 2/2019 was released on March 15th 2019. Its digital version will be available immediately.

Architectural and scenic lighting
The architectural lighting of Bečov nad Teplou castle
Lighting design in a nutshell – Part 41
The analyse of light picture a little more theoretic

Day light
Biggest mistakes in day lighting design of buildings

Fluorescent holography - Upending the world of biological imaging

26.10.2016 | Colorado State University | source.colostate.edu

Optical microscopy experts at Colorado State University are once again pushing the envelope of biological imaging. They have designed and built a fluorescence-detection microscope that combines three-dimensional and high-resolution image processing that’s also faster than comparable techniques.

This new microscope builds upon a previously published technique, and permits digital re-focus of fluorescent light. It illuminates not one point, but multiple points by harnessing delocalized illumination spread over a large area. The physical principles they are using are similar to holography, in which scattered light is used to build a 3-D image.

Fluorescent holography

Using a large illumination field, followed by back-end signal processing, the microscope can define distinct light modulation patterns of many points within the field of view. It builds up a 3-D image by combining the signals from all those distinct patterns.

What does this new technique allow? Deep-tissue images in three dimensions, with better depth of field than comparable techniques. Depth of field, like in photography, means background images are in sharp focus along with the main image. And the CSU researchers can work at 600 frames per second, which is many times faster than established techniques.

Read more at Colorado State University

Image Credit: Colorado State University

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